The holders were bounced out by non-league Macclesfield in the third round - just weeks before Guehi left Palace for Manchester City.
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Jeffers said on the Wayne Rooney Show: “I will say it and I don’t know if I should be saying it but Marc Guehi and Glasner going at it in the tunnel.
“It was at half-time and they were going at it, I walked past them and was like, there is an edge to that.
”I told my lads that you see, you have clearly rattled them.”
Along with Guehi, Glasner has also announced he will leave Palace when his contract expires at the end of June.