Jaden and Reigan Heskey were both second-half substitutes in City's Carabao Cup win at Huddersfield Town.
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The brothers are the sons of former Liverpool, Aston Villa and Leicester City striker Emile Heskey.
Guardiola said after the 2-0 third round win: "Today, six players from the academy made the starting XI.
"Two more came on, the Heskey brothers. I think their father and mother will be so proud of their kids. That's really cool."
Jaden, 19, was given14 minutes, replacing fellow academy graduate Nico O'Reilly, with Reigan, 17, coming on for Savinho shortly after.
