Two different supporter marches were held, with both finishing outside the director's entrance to London stadium.
One of the two organisers, Hammers United, have called for co-owner David Sullivan and chief exec Karren Brady to resign.
They said: "If we are going to get our club back our fanbase is going to have to fight. With Brady and Sullivan at the helm our club is going to die. It is in serious decline and dying a slow death.
"With thousands of long-term, time-served fans walking away, another relegation fight beckoning and another early cup exit, we cannot allow this to happen on our watch.
"We must show the world that Brady and Sullivan's position is untenable and they must step aside for the good of the club.
"They must have no more involvement in the running of West Ham United, which must be handed over to professionals with the expertise and drive to move the club forward."