The Everton midfielder, on-loan from Manchester City, was again ignored for this week's England squad.
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Tuchel's decision coming despite Grealish's outstanding form for Everton this season.
"Jack Grealish may as well book his holiday for the summer now because he's not getting in the squad," Rooney said on the Wayne Rooney Show.
"You think Andy Gordon dropped out and he still doesn't get called into it, so I think Thomas Tuchel is quite set."
Grealish last featured for the Three Lions in October 2024, having been brought back into the fold under temporary boss Lee Carsely after being omitted from Southgate's Euro 2024 squad.