Hansi Flick’s side may have been hit with another major injury ahead of the first El Clasico of the season on Sunday (October 26).
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According to AS, De Jong, 28, who was expected to start alongside Pedri in centre midfield, missed Thursday’s training session.
Barcelona will continue to monitor his situation, but he is now expected to miss the game against arch-rivals Real Madrid.
The current La Liga holders are also sweating over the fitness of Raphinha and Ferran Torres, with Dani Olmo also expected to be out.
