Zubimendi, 27, has left Spain's camp ahead of Tuesday's friendly with Egypt carrying a knee injury.
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The federation announced today: “Martín Zubimendi has withdrawn from the Spanish national team squad due to pain in his right knee.
"In order to avoid any risk and to protect the player's health, it has been decided to remove him from the squad.
"Arsenal FC's medical staff have been informed of the entire process."