The Saints were found guilty of spying on opponents throughout the season after admitting to filming training sessions of opponents Oxford United, Ipswich Town and Middlesbrough.
They were consequently kicked out of the play-off final by the EFL but then appealed the decision on the grounds that the punishment was ‘disproportionate.’
Southampton’s appeal to overturn their punishment has been unsuccessful, meaning Middlesbrough will face Hull in the play-off final on Saturday (May 23).
The independent disciplinary commission also awarded Saints a four-point deduction for the 2026/27 season.
Southampton have now released a statement saying how disappointed they are with the decision.
“This is an extremely disappointing outcome for everybody connected with Southampton Football Club,” it read.
“We know how painful this moment will be for our supporters, players, staff, commercial partners and the wider community who have given so much backing to the team throughout the season and we apologise once again to everyone impacted by this.
“While we fully acknowledge the seriousness of this matter and the scrutiny that has followed, the club has consistently believed the original sporting sanction was disproportionate, a view that has been widely shared by many in the football community over the last 24 hours.
“We would like to place on record our sincere thanks to our supporters for the support, patience and loyalty they have shown throughout an incredibly difficult period. We will share information as soon as possible in relation to ticket refunds for those who bought tickets to Wembley.
“Southampton Football Club has a proud history and strong foundations, but it is clear that trust now needs to be rebuilt. That work begins immediately. The club will reflect carefully on the events that have led to this point, learn from them and take the necessary steps to move forward responsibly.
“While tonight is a painful moment, this football club will respond with humility, accountability and determination to put things right.”