City were due to train on Tuesday in front of the media, but the session was canceled by Guardiola.
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Instead, the players will assemble tomorrow morning ahead of meeting Dortmund, with no pre-match training session held.
The Manchester Evening News says Guardiola's decision is a first for City in European competition this century. With tomorrow's tie against Dortmund being the first without a training session the previous day.
The players worked at the City Football Academy on Monday for a training and recovery session after defeating Bournemouth on Sunday.
It's been suggested Guardiola made the decision to cancel today's session to aid the recovery of his senior players.